DNA Extraction.

Tomorrow we´d like to do a lab practice: DNA Extraction. (include any image).

Before that, as a practice preparation,  I ´d like you to answer these questions (answer as a comment to this post).

1- DNA extraction. What is it?

2- What materials do we need?

3- How to do it?

4- Links to get more information.

5- Any video to clarify the process

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  1. #1 by belenmercader on February 7, 2011 - 1:05 pm

    2. Materials for a DNA extraction:

    · DNA source (such green split peas)
    · Salt
    · Cold water
    · Strairner
    · Enzymes ( pinnaple juice)
    · Alcohol

    We love you teacher (:

  2. #2 by Andrea y Samuel on February 7, 2011 - 1:05 pm

    We answered the point number 5.

  3. #3 by inesrubio on February 7, 2011 - 1:08 pm

    Martin Ramos and Ines Rubio 🙂

    3. How to do it:

    Procedures

    1. Add kiwi/strawberry fruit into extraction solution in the zipper bag. Close bag and squeeze out air.

    2. Crush the kiwi/strawberry thoroughly for 5 minutes. CAREFUL don’t break the bag!

    3. Place the bags into the hot water bath for about 10-15 minutes, making sure the fruit solution is fully beneath the water line. Occasionally shake the bag to evenly distribute the heat.

    4. Move the “mashed” bags of kiwi/strawberry fruit solution into the ice bath for 1 minute. Remove and carefully mix the kiwi/strawberry fruit solution again. Repeat this procedure 5 times.

    5. Tape the cheese cloth over the beakers. Filter the fruit mixture through the cheese cloth. Combine solutions from all student groups at this point. Let the solution drain 5 minutes.

    6. Using the large transfer pipettes, aliquot approximately 2 ml of the kiwi/strawberry fruit solution into a test tube, one for each pair of students.

    7. Add approximately 2 ml of ice-cold ethanol to each tube by dropping it slowly down the side of the test tube, allowing it to rest on top of the kiwi/strawberry fruit mixture. Do not agitate the solution.

    8. Let the solution sit for two minutes without disturbing it. The DNA will appear as transparent, slimy, white mucus which can be spooled up with the wood applicator stick.

  4. #4 by silvia y beatriz on February 7, 2011 - 1:09 pm

    THIS IS THE BEST LINK that shows a DNA extraction ( lab practise interactive)
    http://learn.genetics.utah.edu/content/labs/extraction/
    SOME OTHER ( with theory)
    http://en.wikipedia.org/wiki/DNA_extraction
    http://serc.carleton.edu/microbelife/research_methods/genomics/dnaext.html

    WE LOVE BIOLOGY

  5. #5 by tamara y pilar on February 7, 2011 - 1:09 pm

    1- DNA extraction. What is it?:
    Is the process to collect DNA for it analysis and see DNA molecules.

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